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Recombinant Human IL-36 Receptor Antagonist Protein/IL-36RN/IL-1F5
SKU: PKSH033872-50
Recombinant Human IL-36 Receptor Antagonist Protein/IL-36RN/IL-1F5
| SKU # | PKSH033872 |
| Expression Host | E.coli |
Description
| Synonyms | FIL, UNQ, PRO, IL1F, IL1L, IL1HY, IL1RP, PSORS, IL36RN, FIL1, FIL1(DELTA), FIL1D, IL-36Ra, IL1F5, IL1HY1, IL1L1, IL1RP3, IL36RA, PSORP, PSORS14, UNQ1896, PRO4342, FIL1 Delta, IL-1 Delta, IL-1F5, IL-1HY1, IL-1L1, IL-1ra Homolog 1, IL-1-Related Protein 3, IL-1RP3, Interleukin-1 Delta, Interleukin-1 Family Member 5, Interleukin-1 HY1, Interleukin-1 Receptor Antagonist Homolog 1, Interleukin-1-Like Protein 1, Interleukin-36 Receptor Antagonist Protein |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Val2-Asp155 |
| Accession | Q9UBH0 |
| Calculated Molecular Weight | 16.8 kDa |
| Observed Molecular Weight | 17 kDa |
| Tag | None |
| Bio-activity | Measured by its ability to inhibit IL-36 alpha, IL-36 beta or IL-36 gamma-induced IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 23.14 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Human Interleukin-36 Receptor Antagonist (IL-36RN) is a secreted protein which belongs to the Interleukin 1 cytokine family (IL-1 family). IL-36RN is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RN is also detected in the spleen, brain leukocyte and macrophage cell types. Increased in lesional psoriasis skin. IL-36RN is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to Interleukin 1 family member 9 (IL1F9). Dysregulated expression of novel agonistic and antagonistic IL-1 family member ligands can promote cutaneous inflammation, revealing potential novel targets for the treatment of inflammatory skin disorders. Human and mouse IL-36RN share 90% sequence identity.