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Recombinant Human IL10-RA/IL-10 Rα Protein (His Tag)
SKU: PKSH033632-50
Recombinant Human IL10-RA/IL-10 Rα Protein (His Tag)
| SKU # | PKSH033632 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | IL-10R subunit, Interleukin-10 receptor subunit, IL-10R, IL10RA, CD210, CD210a, CDW210A, HIL-10R, IL-10R1, IL10R, IL-10 receptor subunit alpha, IL-10R subunit 1, IL-10R subunit alpha, IL-10RA, Interleukin-10 receptor subunit 1, Interleukin-10 receptor subunit alpha, IL-10R1, IL-10Ra, CDw210a antigen, HIL 10R, HIL10R, I10R1, IL 10R, IL 10R 1, IL 10R A, IL 10R1, IL10R1, Interleukin 10 receptor alpha, Interleukin 10 receptor alpha chain |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | His22-Asn235 |
| Accession | Q13651 |
| Calculated Molecular Weight | 25.2 kDa |
| Observed Molecular Weight | 38-59 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin-10 Receptor alpha (IL-10Rα) is a transmembrane glycoprotein member of the class II cytokine receptor family. Mature human IL-10 Rα consists of a 214 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain. Within the ECD, human IL-10 Rα shares 59% aa sequence identity with mouse and rat IL-10Rα. IL-10 Rα is required for mediating the effects of IL-10,a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer. IL-10Rα is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 Rα, resulting in the recruitment of IL-10 Rβ. Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection. Polymorphisms of human IL-10 Rα may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL10.