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Recombinant Human IL1F5/IL36RN Protein
SKU: PKSH031850-100
Recombinant Human IL1F5/IL36RN Protein
| SKU # | PKSH031850 |
| Expression Host | E.coli |
Description
| Synonyms | FIL, UNQ, PRO, IL1F, IL1L, IL1HY, IL1RP, PSORS, IL36RN, FIL1, FIL1(DELTA), FIL1D, IL-36Ra, IL1F5, IL1HY1, IL1L1, IL1RP3, IL36RA, PSORP, PSORS14, UNQ1896, PRO4342, FIL1 Delta, IL-1 Delta, IL-1F5, IL-1HY1, IL-1L1, IL-1ra Homolog 1, IL-1-Related Protein 3, IL-1RP3, Interleukin-1 Delta, Interleukin-1 Family Member 5, Interleukin-1 HY1, Interleukin-1 Receptor Antagonist Homolog 1, Interleukin-1-Like Protein 1, Interleukin-36 Receptor Antagonist Protein |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Asp155 |
| Accession | Q9UBH0 |
| Calculated Molecular Weight | 17.8 kDa |
| Observed Molecular Weight | 17 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to inhibit IL-36 gamma-induced IL-8 secretion in PBMC cells.The ED50 for this effect is <2 ng/mL in the presence of 500 ng/mL of recombinant human IL-36 gamma. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin-1 family member 5 (IL-1F5); also known as interleukin 36 receptor antagonist (IL36RA); is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family; member 6 (IL1F6). IL-1F5 is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9). IL-1F5 could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A); beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1); which is present in epithelial barriers and takes part in local inflammatory response. It has been proved that IL-1F5 induces IL-4 mRNA and protein expression in glia in vitro and enhances hippocampal expression of IL-4 following intracerebroventricular injection. The inhibitory effect of IL-1F5 on LPS-induced IL-1β is attenuated in cells from IL-4-defective mice. Experiment results suggest that IL-1F5 mediates anti-inflammatory effects through its ability to induce IL-4 production and that this is a consequence of its interaction with the orphan receptor; single Ig IL-1R-related molecule (SIGIRR)/TIR8; as the effects were not observed in SIGIRR−/− mice. In contrast to its effects in brain tissue; IL-1F5 did not attenuate LPS-induced changes; or up-regulated IL-4 in macrophages or dendritic cells; suggesting that the effect is confined to the brain.