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Recombinant Human IL27Ra/TCCR/WSX1 Protein (Fc Tag)(Active)
SKU: PKSH031571-100
Recombinant Human IL27Ra/TCCR/WSX1 Protein (Fc Tag)(Active)
| SKU # | PKSH031571 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CRL, WSX, UNQ, PRO, zcytor, IL27RA, CRL1, IL-27RA, IL27R, TCCR, WSX1, zcytor1, UNQ296, PRO336 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Arg 514 |
| Accession | NP_004834.1 |
| Calculated Molecular Weight | 79.0 kDa |
| Observed Molecular Weight | 105-115 kDa |
| Tag | C-hFc |
| Bio-activity | Immobilized human IL27 at 20 μg/ml (100 μl/well) can bind human IL27Ra with a linear ranger of 0.064-1. 6 μg/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4, 8% trehalose, 8% mannitol Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
The interleukin-27 receptor is a type I cytokine receptor for interleukin-27. It is a heterodimer composed of the interleukin 27 receptor, alpha subunit and glycoprotein 130. WSX-1/IL-27R, a class I cytokine receptor that is homologous to the β2 chain of the IL-12R in both sequence and structure, is highly expressed by resting/naive CD4+ T cells and CD8+ T cells. WSX-1/IL-27R belongs to the IL-6/IL-12 family of cytokines and induced proliferation of naive CD4(+) T cells and the generation of a Th1-type adaptive immune response. WSX-1/IL-27R functions as a receptor for IL27. IL-27 not only contributes to the development of an adaptive immune response through its action on CD4(+) T cells, it also directly acts on cells of the innate immune system. WSX-1/IL-27R requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. It is involved in the regulation of Th1-type immune responses. IL-27R also appears to be involved in innate defense mechanisms. IL27RA is essential for transcriptional activation of STAT1 and for augmenting the induction of T-bet expression during initiation of Th1 cell differentiation.