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Recombinant Human IL4RA/CD124 Protein (Fc Tag)(Active)
SKU: PKSH032648-50
Recombinant Human IL4RA/CD124 Protein (Fc Tag)(Active)
| SKU # | PKSH032648 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | 582J, IL4R, CD124, IL-4RA, IL4RA, IL-4 receptor subunit alpha, IL-4-binding protein, IL4-BP, IL-4R subunit alpha, IL-4R-alpha, Interleukin-4 receptor subunit alpha, 582J2.1, IL-4R&, alpha, IL 4R alpha, IL4R nirs variant 1, IL4RA, Interleukin 4 receptor, Interleukin 4 receptor alpha chain, sIL4Ralpha/prot, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, Soluble interleukin-4 receptor subunit alpha |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met26-Gln231 |
| Accession | P24394 |
| Calculated Molecular Weight | 50.2 kDa |
| Observed Molecular Weight | 60-70 kDa |
| Tag | C-Fc |
| Bio-activity | Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 5-20 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin 4 Receptor alpha (IL4-Ra) is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. Mature human IL4-Ra consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a cytokine binding region and one fibronectin type III domain; a 24 aa transmembrane segment; and a 569 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif. IL4-Ra plays an important role in Th2-biased immune responses; alternative macrophage activation; mucosal immunity; allergic inflammation; tumor progression; and atherogenesis. Soluble forms of IL4-Ra; generated by alternate splicing or proteolysis; retain ligand binding properties and inhibit IL-4 bioactivity. IL4-Ra is a component of two distinct receptor complexes and shows species selectivity between human and mouse. It can associate with the common gamma chain (γc) to form the IL-4 responsive type I receptor in which γc increases the affinity for IL-4 and enables signaling. It can alternatively associate with IL13-Ra1 to form the type II receptor which is responsive to both IL-4 and IL-13. The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other cytokines that utilize γc.