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Recombinant Human Interleukin-5/IL-5 Protein (His Tag)(Active)
SKU: PKSH032653-50
Recombinant Human Interleukin-5/IL-5 Protein (His Tag)(Active)
| SKU # | PKSH032653 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | nterleukin, Interleukin, IL5, EDF, IL-5, TRF, BCDFII, B-cell differentiation factor I, B-cell growth factor I, BCGF-II, Cytotoxic T-lymphocyte inducer, E-CSF, Eosinophil differentiation factor, ESGF, Interleukin-5, nterleukin-5, T-cell replacing factor |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ile20-Ser134 |
| Accession | P05113 |
| Calculated Molecular Weight | 14.2 kDa |
| Observed Molecular Weight | 20 kDa |
| Tag | C-His |
| Bio-activity | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.5 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
IL-5 is expressed in eosinophils, NK cells, TC2 CD8+ T cells, mast cells, CD45+ CD4+ T cells, gamma delta T cells and IL-1 beta activated endothelial cells. IL-5 acts as a growth and differentiation factor for both B cells and eosinophils. Relative to B cells, IL-5 appears to induce the differentiation of activated conventional B-2 cells into Ig-secreting cells. In addition, it induces the growth of B-1 progenitors as well as IgM production by B-1 cells.IL-5 appears to perform a number of functions on eosinophils. These include the down modulation of Mac-1,the upregulation of receptors for IgA and IgG,the stimulation of lipid mediator (leukotriene C4 and PAF) secretion and the induction of granule release.IL-5 also promotes the growth and differentiation of eosinophils.