Recombinant Human ITGA5 Protein (His Tag)(Active)
SKU: PKSH032600-50
Recombinant Human ITGA5 Protein (His Tag)(Active)
SKU # | PKSH032600 |
Expression Host | HEK293 Cells |
Description
Synonyms | CD49 Antigen-Like Family Member E, CD49e, FNRA, Fibronectin Receptor Subunit Alpha, ITGA5, Integrin Alpha-5, Integrin Alpha-F, VLA-5 |
Species | Human |
Expression Host | HEK293 Cells |
Sequence | Phe42-Tyr995 |
Accession | P08648 |
Calculated Molecular Weight | 105.1 kDa |
Observed Molecular Weight | 130 kDa |
Tag | C-His |
Bio-activity | Not validated for activity |
Properties
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background
Integrin α-5 belongs to the Integrin α chain family and contains 7 FG-GAP repeats. Integrin α-5 joins with Integrin-β1 to form a fibronectin and laminin receptor which recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection; the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. It is expressed on fibroblasts; endothelial cells; peripheral T cells and platelets. Integrin α-5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains. In addition to adhesion; ITGA5 participates in cell-surface mediated signalling.