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Recombinant Human JAM-A/F11R Protein (His Tag)(Active)
SKU: PKSH031771-100
Recombinant Human JAM-A/F11R Protein (His Tag)(Active)
| SKU # | PKSH031771 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD321, F11R, JAM-1, JAM-A, JAM1, JAMA, JCAM, Junctional Adhesion Molecule 1, Junctional Adhesion Molecule A, KAT, PAM-1, Platelet Adhesion Molecule 1, Platelet F11 Receptor |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Ala 242 |
| Accession | NP_058642.1 |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 28-32 kDa |
| Tag | C-His |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 x 104cells/well are added to JAM-A-Fc coated plates (2. 5μg/mL, 100 μL/well)in the presence of 20 ng/mL PMA, approximately 30-40% will adhere after 30 minutes at 37℃. |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Junctional adhesion molecule-A (JAM-A); also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321); is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells; as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1; involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily; JAM-A protein involves in platelet adhesion; secretion and aggregation; and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition; it may be a potential therapeutic target for breast cancer.