Total Price: $0.00
Contact Information
Recombinant Human LAG3/CD223 Protein (His Tag)(Active)
SKU: PKSH033282-50
Recombinant Human LAG3/CD223 Protein (His Tag)(Active)
| SKU # | PKSH033282 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD223, LAG-3, LAG3, Lymphocyte activation gene 3 protein, Protein FDC |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Leu23-Gly434 |
| Accession | P18627 |
| Calculated Molecular Weight | 45.5 kDa |
| Observed Molecular Weight | 60-80 kDa |
| Tag | C-His |
| Bio-activity | Loaded Anti-Human LAG-3 mAb-Fc on Protein A Biosensor, can bind Human LAG-3-6His with an affinity constant of 0.46 nM as determined in BLI assay. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Human Lymphocyte activation gene 3 protein( LAG3) is a member of immunoglobulin (Ig) superfamily. LAG3 contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. LAG3 is involved in lymphocyte activation and can bind to HLA class-II antigens. It is selectively expressed in activated T and NK cells. LAG3 has a negative regulatory function in T cells and acts as as a new marker of T cell induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signaling. It can lead to increased antigen-specific T-cell responses in vivo. LAG-3 has higher affinity to MHC class II than CD4.