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Recombinant Human LRAP/ERAP2 Protein (His Tag)(Active)
SKU: PKSH031454-50
Recombinant Human LRAP/ERAP2 Protein (His Tag)(Active)
| SKU # | PKSH031454 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | FLJ23633, FLJ23701, FLJ23807, L-RAP, LRAP, LRAP.ERAP2 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ala 56-Thr 960 |
| Accession | NP_071745.1 |
| Calculated Molecular Weight | 106 kDa |
| Observed Molecular Weight | 115-125 kDa |
| Tag | N-His |
| Bio-activity | Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (Arg-AMC). The specific activity is > 50 pmoles/min/μg. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 12.5mM Tris, 75mM NaCl, pH 7.5, 50% glycerol Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Leukocyte-derived arginine aminopeptidase (LRAP), also known as endoplasmic reticulum-aminopeptidase 2 (ERAP2), is the second identified aminopeptidase localized in the in the lumenal side of endoplasmic reticulum (ER) processing antigenic peptides presented to major histocompatibility complex (MHC) class I molecules. It is a 960-amino acid protein with significant homology to placental leucine aminopeptidase and adipocyte-derived leucine aminopeptidase. LRAP preferentially hydrolyzes the basic residues Arg and Lys, and contains the HEXXH(X)18E zinc-binding motif, which is the characteristic of the M1 family of zinc metallopeptidases which also includes PILS/ARTS1/ERAP1 and LNPEP/PLAP. Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors.