Total Price: $0.00
Contact Information
Recombinant Human LSM4 Protein (His Tag)
SKU: PKSH033173-50
Recombinant Human LSM4 Protein (His Tag)
| SKU # | PKSH033173 |
| Expression Host | E.coli |
Description
| Synonyms | GRP, Glycine-Rich Protein, LSM4, U6 snRNA-Associated Sm-Like Protein LSm4 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Gln139 |
| Accession | Q9Y4Z0 |
| Calculated Molecular Weight | 17.5 kDa |
| Observed Molecular Weight | 17 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, pH 8.0 . Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
U6 snRNA-associated Sm-like protein LSm4 (LSM4) is a member of the snRNP Sm proteins family. Sm-like proteins contain the Sm sequence motif and are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. LSM4 forms a heteromer with a donut shape. The complexes are involved in various steps of RNA metabolism. LSM4 binds specifically to the 3-terminal U-tract of U6 snRNA. LSM4 contributes RNA protein interactions and structural changes which are essential during ribosomal subunit assembly.