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Recombinant Human MERTK/MER Protein (His Tag)
SKU: PKSH033481-50
Recombinant Human MERTK/MER Protein (His Tag)
| SKU # | PKSH033481 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | MER, MERTK, Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK, Tyrosine-protein kinase Mer |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met1-Ala323 |
| Accession | Q1RMG3 |
| Calculated Molecular Weight | 36.0 kDa |
| Observed Molecular Weight | 60-120 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Tyrosine-protein kinase Mer (MERTK) is a single-pass type I membrane protein which belongs to the MER/AXL/TYRO3 receptor kinase family. MERTK include two fibronectin type-III domains; two Ig-like C2-type domains; and one tyrosine kinase domain. It can’t be expressed in normal B- and T-lymphocytes; but it is usually expressed in numerous neoplastic B- and T-cell lines. MERTK could regulate many physiological processes; such as cell survival; migration; differentiation. It was demonstrated that the MERTK plays critical role in the engulfment and efficient clearance of apoptotic cells; platelet aggregation; and cytoskeleton reorganization. Not only these; it also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1; which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. In addition; MERTK could regulate rod outer segments fragments phagocytosis in the retinal pigment epithelium (RPE); deficiency in MERTK are the cause of retinitis pigmentosa.