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Recombinant Human Mesothelin/MSLN Protein (aa 37-286, His Tag)
SKU: PKSH032746-50
Recombinant Human Mesothelin/MSLN Protein (aa 37-286, His Tag)
| SKU # | PKSH032746 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CAK1, CAK1 antigen, MPF, MSLN, Megakaryocyte potentiating factor, Pre-pro-megakaryocyte-potentiating factor, SMR, mesothelin, soluble MPF mesothelin related protein |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Leu37-Arg286 |
| Accession | Q13421 |
| Calculated Molecular Weight | 27.8 kDa |
| Observed Molecular Weight | 30-35 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Mesothelin is a cell surface glycoprotein whose expression is limited to mesothelial cells of the serosa (pleura; pericardium; and peritoneum) and epithelial cells of the trachea; tonsils; fallopian tube; and kidneys. Mesothelin plays an important role in cell survival; proliferation; migration; invasion; tumor progression; and resistance to chemotherapy. The overexpression of mesothelin can activate NF-κB and signal transducer and activator of transcription 3 (Stat3); inhibit apoptotic signaling and TNF-α-induced apoptosis; and accelerate the G1–S transition. Mesothelin is also found overexpressed in various cancers; including malignant mesothelioma; pancreatic or ovarian carcinoma; sarcomas and in some gastrointestinal or pulmonary carcinomas. As a result of its limited expression in normal tissues; mesothelin has been reported as an ideal tumor-associated marker for the development of targeted therapy.