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Recombinant Human MICA Protein (Fc Tag)(Active)
SKU: PKSH032753-50
Recombinant Human MICA Protein (Fc Tag)(Active)
| SKU # | PKSH032753 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | MHC Class I Polypeptide-Related Sequence A, MIC-A, MICA, PERB11.1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Glu24-Gln308 |
| Accession | AAH16929.1 |
| Calculated Molecular Weight | 59.9 kDa |
| Observed Molecular Weight | 85-110 kDa |
| Tag | C-Fc |
| Bio-activity | Immobilized Mouse NKG2D at 2 μg/ml (100 μl/well) can bind Human MICA (C-Fc). The EC50 of Human MICA (C-Fc) is ≤10 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
MHC class I polypeptide-related sequence A, also known as MIC-A, PERB11.1 and MICA, is a single-pass type I membrane protein which belongs to the MHC class I family of MIC subfamily. MICA contains one Ig-like C1-type domain and is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA is the ligand for NK cell activating receptor KLRK1/NKG2D. MICA seems to have no role in antigen presentation. MICA leads to cell lysis by binding to KLRK1.