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Recombinant Human MICA Protein (His Tag)(Active)
SKU: PKSH033333-50
Recombinant Human MICA Protein (His Tag)(Active)
| SKU # | PKSH033333 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | MHC Class I Polypeptide-Related Sequence A, MIC-A, MICA, PERB11.1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Glu24-Gln308 |
| Accession | AAH16929.1 |
| Calculated Molecular Weight | 33.9 kDa |
| Observed Molecular Weight | 60 kDa |
| Tag | C-His |
| Bio-activity | Loaded Human NKG2D-Fc on Protein A Biosensor, can bind Human MICA-His with an affinity constant of 24.6 nM as determined in BLI assay. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
MHC Class I Polypeptide-Related Sequence A (MICA) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. Unlike classical MHC class I molecules, MICA does not form a heterodimer with beta-2-microglobulin. MICA shares 85% amino acid identity with a closely related protein, MICB. MICA acts as a stress-induced self-antigen that is recognized by NK cells, NKT cells, and most of the subtypes of T cells. As a Ligand for the KLRK1/NKG2D receptor, MICA binds to KLRK1 leads to cell lysis. MICA functions as an antigen for gamma delta T cells and is frequently expressed in epithelial tumors. MICA antigens are able to elicit the synthesis of alloantibodies in transplant recipients. Studies have shown that anti-MICA antibodies are associated with acute renal allograft rejection and failure. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.