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Recombinant Human MMP-3 Protein (Active)
SKU: PKSH031588-20
Recombinant Human MMP-3 Protein (Active)
| SKU # | PKSH031588 |
| Expression Host | E.coli |
Description
| Synonyms | CHDS6, MMP-3, MMP3, Matrix metalloproteinase-3, SL-1, STMY, STMY1, STR1, Stromelysin-1, Transin-1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Tyr 18-Thr 272 |
| Accession | AAA36321.1 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 34 kDa |
| Tag | None |
| Bio-activity | Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nva-WR-K(Dnp)-NH2, AnaSpec, Catalog # 27114.The specific activity is > 300 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Chymotrypsin for an activated form) |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 50mM Tris, 10mM CaCL2, 1uM ZnCL2, 50mM NaCl, 0.5% Brij35, pH 7.0 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Matrix metallopeptidase 3 (abbreviated as MMP3) is also known as stromelysin 1 and progelatinase. MMP3 is a member of the matrix metalloproteinase (MMP) family whose members are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes including arthritis and metastasis. As a secreted zinc-dependent endopeptidase, MMP3 exerts its functions mainly in extracellular matrix. This protein is activated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMP3 plays a central role in degrading collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP3 can also active other MMPs such as MMP1, MMP7, and MMP9, rendering MMP3 crucial in connective tissue remodeling. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Synthetic or natural inhibitors of MMPs result in inhibition of metastasis, while up-regulation of MMPs led to enhanced cancer cell invasion.