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Recombinant Human NAD Kinase/NADK Protein (His Tag)
SKU: PKSH032782-50
Recombinant Human NAD Kinase/NADK Protein (His Tag)
| SKU # | PKSH032782 |
| Expression Host | E.coli |
Description
| Synonyms | NAD Kinase, NADK, Poly(P)/ATP NAD Kinase |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ser64-Gly446 |
| Accession | AAH01709.1 |
| Calculated Molecular Weight | 44.4 kDa |
| Observed Molecular Weight | 49 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, pH 7.5. |
| Reconstitution | Not Applicable |
Background
NAD Kinase (NADK) is an enzyme that belongs to the NAD Kinase family. It is a widely expressed enzyme, but it is not detected in skeletal muscle. NADK converts Nicotinamide Adenine Dinucleotide (NAD+) into NADP+, through phosphorylating the NAD+ coenzyme. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. The structure of the NADK from the archaean Archaeoglobus fulgidus has been determined.