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Recombinant Human NCK1 Protein (His Tag)
SKU: PKSH032339-50
Recombinant Human NCK1 Protein (His Tag)
| SKU # | PKSH032339 |
| Expression Host | E.coli |
Description
| Synonyms | Cytoplasmic Protein NCK1, NCK, NCK adaptor Protein 1, NCK1, Nck-1, SH2/SH3 Adaptor Protein NCK-Alpha |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Ser377 |
| Accession | P16333 |
| Calculated Molecular Weight | 45.0 kDa |
| Observed Molecular Weight | 50 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 80 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 5% Mannitol, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Cytoplasmic Protein NCK1 (NCK1) is a cytoplasmic protein that contains one SH2 domain and three SH3 domains. NCK1 is a member of the adapter family, which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 plays a role in the DNA damage response, but not in the detection of the damage by ATM/ATR. It is also involved in transducing signals from receptor tyrosine kinases to downstream signal recipients, such as ELK1-dependent transcriptional activation in response to activated Ras signaling.