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Recombinant Human β-NGF/NGFB Protein (aa 122-241, E. coli)(Active)
SKU: PKSH033269-100
Recombinant Human β-NGF/NGFB Protein (aa 122-241, E. coli)(Active)
| SKU # | PKSH033269 |
| Expression Host | E.coli |
Description
| Synonyms | Beta-NGF, Beta-Nerve Growth Factor, NGF, NGFB |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ser122-Ala241 |
| Accession | P01138 |
| Calculated Molecular Weight | 14.4 kDa |
| Observed Molecular Weight | 11 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <0.7 ng/mL. The specific activity of recombinant human beta-NGF is > 1 x 106 IU/mg. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α; β; and γ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF; NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF; and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine; which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity.