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Recombinant Human β-NGF/NGFB Protein (with pro peptide)(Active)
SKU: PKSH033552-50
Recombinant Human β-NGF/NGFB Protein (with pro peptide)(Active)
| SKU # | PKSH033552 |
| Expression Host | E.coli |
Description
| Synonyms | Beta-NGF, Beta-Nerve Growth Factor, NGF, NGFB |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Glu19-Ala241 |
| Accession | P01138 |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 30 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl,500mM NaCl,5%Trehalose,5%Mannitol,0.01%Tween 80,1mM EDTA,pH8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystin knot motif consisting of three cystine bridges; whereas proneurotrophins and mature neurotrophins elicit opposite biological effects. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration.