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Recombinant Human PACT protein (His tag)
SKU: PDEH100367-100
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Recombinant Human PACT protein (His tag)
| SKU # | PDEH100367 |
| Expression Host | E. coli |
Description
| Synonyms | Interferon-inducible double-stranded RNA-dependent protein kinase activator A;PRKRA;PKR-associated protein X |
| Species | Human |
| Expression_host | E.coli |
| Sequence | Met 1-Lys 313 |
| Accession | O75569 |
| Mol_Mass | 34.3 kDa |
| AP_Mol_Mass | 38 kDa |
| Tag | N-His & C-His |
| Bio_Activity | Not validated for activity |
Propertie
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
Activates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1.