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Recombinant Human PCSK9 Protein (D374Y, His Tag)
SKU: PKSH032947-50
Recombinant Human PCSK9 Protein (D374Y, His Tag)
| SKU # | PKSH032947 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | NARC-1, NARC1, Neural Apoptosis-Regulated Convertase 1, PC9, PCSK9, Proprotein Convertase 9, Proprotein Convertase Subtilisin/Kexin Type 9, Subtilisin/Kexin-Like Protease PC9 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gln31-Gln692(Asp374Tyr,Val474Ile,Gly504Arg,Gly670Glu) |
| Accession | Q8NBP7 |
| Calculated Molecular Weight | 15-18&60-80 kDa |
| Observed Molecular Weight | 13.8&58.2 kDa |
| Tag | C-His |
| Bio-activity | Immobilized Recombinant Human LDL R (C-Fc) at 5μg/ml (100 μl/well) can bind Recombinant Human PCSK9 . The ED50 of Recombinant Human PCSK9 is 39.17 ng/ml. |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 20% Glycerol, pH 7.4. |
| Reconstitution | Not Applicable |
Background
Recombinant Human Proprotein Convertase Subtilisin/Kexin Type 9/PCSK9 (D374Y) is a gain of function mutant of human PCSK9 protein. Human PCSK9 is a secretory subtilase belonging to the proteinase K subfamily. PCSK9 is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the ER, the pro domain and mature chain are secreted together through noncovalent interactions. PCSK9 binds with low-density lipoprotein receptor (LDLR) and it plays a major regulatory role in cholesterol homeostasis. Inhibition of PCSK9 function by preventing PCSK9/LDLR interaction is currently being explored as a means of lowering cholesterol levels. PCSK9 also binds to apolipoprotein receptor 2 (ApoER2), and play a role in the neural development.