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Recombinant Human PFK1/PFKM Protein (His & GST Tag)
SKU: PKSH030320-50
Recombinant Human PFK1/PFKM Protein (His & GST Tag)
| SKU # | PKSH030320 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | 6-phosphofructokinase, ATP-PFK, GSD7, PFK-1, PFK1, PFKA, PFKM, PFKX, PPP1R122, Phosphofructo-1-kinase isozyme A, Phosphofructokinase 1, Phosphohexokinase, muscle type |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Thr 2-Val 780 |
| Accession | P08237-1 |
| Calculated Molecular Weight | 112.9 kDa |
| Observed Molecular Weight | 113 kDa |
| Tag | N-His-GST |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol |
| Reconstitution | Not Applicable |
Background
PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related with glycogen storage disease type VII, also identified as Tarui disease.