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Recombinant Human PPP3R1 Protein (His Tag)(Active)
SKU: PKSH030576-100
Recombinant Human PPP3R1 Protein (His Tag)(Active)
| SKU # | PKSH030576 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | CNA2, CNB, Calcineurin Subunit B Type 1, PPP3R1, Protein Phosphatase 2B Regulatory Subunit 1, Protein Phosphatase 3 Regulatory Subunit B Alpha Osoform 1 |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Gly2-Val170 |
| Accession | P63098 |
| Calculated Molecular Weight | 21.4 kDa |
| Observed Molecular Weight | 20 kDa |
| Tag | N-His |
| Bio-activity | Using the Octet RED System, the affinity constant (Kd) of human PPP3R1-His bound to Human PPIA-His was 6 nM. |
Properties
| Purity | > 87 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol, 2mM DTT Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
PPP3R1 belongs to the calcineurin regulatory subunit family. It is a regulatory subunit of calcineurin. Calcineurin is composed of two subunits: calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation; nuclear translocation; and early gene expression in T-cells. PPP3R1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase which takes a vital part in the T cell activation pathway. PPP3R1 is involved in protein dephosphorylation; NFAT protein import into nucleus (ortholog) and epithelial to mesenchymal transition (ortholog). It participates in calcineurin signaling pathway; mitogen activated protein kinase signaling pathway. PPP3R1 interacts with (+)-pilocarpine; 2;4-dinitrotoluene and ammonium chloride. It contains four EF-hand domains and four functional calcium-binding sites. PPP3R1 play an improtant role in the T cell activation pathway.