Recombinant Human R-Spondin 1/RSPO1 Protein (aa 1-146, His Tag)(Active)
SKU: PKSH031268-100
Recombinant Human R-Spondin 1/RSPO1 Protein (aa 1-146, His Tag)(Active)
SKU # | PKSH031268 |
Expression Host | HEK293 Cells |
Description
Synonyms | CRISTIN3, FLJ40906, R-spondin, R-spondin1, RP11-566C13.1, RP23-325M14.2, RSPO Rspo1, RSPO1, Roof plate-specific spondin-1, Rspondin |
Species | Human |
Expression Host | HEK293 Cells |
Sequence | Met 1-Ala 146 |
Accession | NP_001033722.1 |
Calculated Molecular Weight | 14 kDa |
Observed Molecular Weight | 23 kDa |
Tag | C-His |
Bio-activity | Measured by its ability to bind recombinant mouse CD36 in a functional ELISA. |
Properties
Purity | > 98 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background
RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.