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Recombinant Human ROR1 Protein (aa 453-783, His & GST Tag)(Active)
SKU: PKSH030321-50
Recombinant Human ROR1 Protein (aa 453-783, His & GST Tag)(Active)
| SKU # | PKSH030321 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | NTRKR1, dJ537F10.1 |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Met 453-Asn783 |
| Accession | AAA60275.1 |
| Calculated Molecular Weight | 65.3 kDa |
| Observed Molecular Weight | 63 kDa |
| Tag | N-His-GST |
| Bio-activity | The specific activity was determined to be 0.3 nmol/min/mg using MBP as substrate. |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, 2mM GSH, 3mM DTT, 10% glycerol, pH 7.4 |
| Reconstitution | Not Applicable |
Background
Receptor tyrosine kinase-like orphan receptor 1 (ROR1), also known as neurotrophic tyrosine kinase, it is a member of the ROR family within receptor tyrosine kinases (RTK) superfamily. Human ROR1 is a type I transmembrane protein with 937 amino acids (aa) in length. It contains a 29 aa signal sequence, a 377 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 510 aa cytoplasmic region. ROR1 expressed strongly in human heart, lung and kidney, but weakly in the CNS. At developmental stage, it expressed at high levels during early embryonic development. ROR1 has been shown to have very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. It may act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling.