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Recombinant Human RSPO3 Protein (Fc & His Tag)
SKU: PKSH033378-50
Recombinant Human RSPO3 Protein (Fc & His Tag)
| SKU # | PKSH033378 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CRISTIN1, PWTSR, Protein with TSP type-1 repeat, R-spondin-3, RSPO3, Roof plate-specific spondin-3, THSD2, Thrombospondin type-1 domain-containing protein 2 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gln22-Val201 |
| Accession | Q9BXY4 |
| Calculated Molecular Weight | 47.9 kDa |
| Observed Molecular Weight | 61 kDa |
| Tag | C-Fc-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
R-spondin-3 (RSPO3); also known as Protein with TSP type-1 repeat; Roof plate-specific spondin-3; Thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2 and CRISTIN1; is a member of the thrombospondin type 1 repeat supergene family. RSPO3 is a secreted protein and widely expressed in many tissues. RSPO3 contains two Furin-like repeats which have been found in a variety of eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases; and one TSP type-1 domain; RSPO3 founctions as a activator of the beta-catenin signaling cascade; leading to TCF-dependent gene activation. Otherwise; RSPO3 may negatively regulate the TGF-beta pathway.