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Recombinant Human Semaphorin 3A/SEMA3A Protein (His & Flag Tag)
SKU: PKSH033535-50
Recombinant Human Semaphorin 3A/SEMA3A Protein (His & Flag Tag)
| SKU # | PKSH033535 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Hsema-I, Hsema-III, SEMA1, SEMA3A, SEMAD, SemD, Sema III, Semad, Semaphorin III, coll-1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Lys26-Phe546 |
| Accession | Q14563 |
| Calculated Molecular Weight | 61.3 kDa |
| Observed Molecular Weight | 70-75 kDa |
| Tag | N-His-Flag |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 100mM NaCl, 0.05% Tween 80, pH 8.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
emaphorin-3A is a secreted protein which belongs to the semaphorin family. Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 500 aa Sema domain.This protein can function as either a chemorepulsive agent; inhibiting axonal outgrowth; or as a chemoattractive agent; stimulating the growth of apical dendrites. In both cases; the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines.