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Recombinant Human SF3B3 protein (GST tag)
SKU: PDEH100430-100
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Recombinant Human SF3B3 protein (GST tag)
| SKU # | PDEH100430 |
| Expression Host | E. coli |
Description
| Synonyms | Splicing factor 3B subunit 3;SF3B3;Spliceosome-associated protein 130 |
| Species | Human |
| Expression_host | E.coli |
| Sequence | Glu 250-Phe 399 |
| Accession | Q15393-3 |
| Mol_Mass | 41.4 kDa |
| AP_Mol_Mass | 42 kDa |
| Tag | N-GST |
| Bio_Activity | Not validated for activity |
Propertie
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.