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Recombinant Human TIM-3/HAVCR2 Protein (His Tag)(Active)
SKU: PKSH033509-50
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Recombinant Human TIM-3/HAVCR2 Protein (His Tag)(Active)
| SKU # | PKSH033509 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | HAVCR2, HAVcr-2, Hepatitis A Virus Cellular Receptor 2, T-Cell Immunoglobulin and Mucin Domain-Containing Protein 3, T-Cell Membrane Protein 3, TIM-3, TIM3, TIMD-3, TIMD3 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ser22-Arg200 |
| Accession | AAL65157.1 |
| Calculated Molecular Weight | 20.9 kDa |
| Observed Molecular Weight | 37 kDa |
| Tag | C-His |
| Bio-activity | Immobilized Anti-Human TIM-3 mAb at 2μg/ml (100 μl/well) can bind Human TIM-3-His. The ED50 of Recombinant Human TIM-3-His is 8.51ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
T-Cell Membrane Protein 3 (TIM3) is a single-pass type I membrane protein that belongs to the TIM family of immunoglobulin superfamily. TIM3 includes a signal sequence (aa 1-21); an extracellular region (aa 22-202) with one Ig-like V-type domain; a transmembrane segment (aa 203-223); and a cytoplasmic domain (aa 224 - 301). TIM3 regulates macrophage activation; inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. It may be also involved in T-cell homing and as a receptor for LGALS9.