Recombinant Human Ub protein (His tag)
SKU: PDEH100199-100
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Recombinant Human Ub protein (His tag)
SKU # | PDEH100199 |
Expression Host | E. coli |
Description
Synonyms | Polyubiquitin-B;UBB;Ubiquitin B;Ubiquitin |
Species | Human |
Expression_host | E.coli |
Sequence | Lys 103-Cys 229 |
Accession | P0CG47 |
Mol_Mass | 13.9 kDa |
AP_Mol_Mass | 15 kDa |
Tag | N-His |
Bio_Activity | Not validated for activity |
Properties
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin level | Please contact us for more information. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
Polyubiquitin-B(UBB) is one of Ubiquitins. Ubiquitin is one of the most conserved proteins known in eukaryotic organisms. Ubiquitin is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.