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Recombinant Human UBE2D4 Protein (GST Tag)
SKU: PKSH033309-50
Recombinant Human UBE2D4 Protein (GST Tag)
| SKU # | PKSH033309 |
| Expression Host | E.coli |
Description
| Synonyms | HBUCE1, UBCH5D, UBE2D4, Ubiquitin Carrier Protein D4, Ubiquitin-Conjugating Enzyme E2 D4, Ubiquitin-Protein Ligase D4 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Met 147 |
| Accession | Q9Y2X8 |
| Calculated Molecular Weight | 43.5 kDa |
| Observed Molecular Weight | 40 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
| Reconstitution | Not Applicable |
Background
Ubiquitin-Conjugating Enzyme E2 D4 (UBE2D4) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2D4 has been proposed to participate in Ubl conjugation pathway. UBE2D4 takes part in post-translational protein modification; protein K6-linked ubiquitination; protein K11-linked ubiquitination; protein K27-linked ubiquitination; protein K29-linked ubiquitination; protein K48-linked ubiquitination; and protein K63-linked ubiquitination. UBE2D4 regulate of protein metabolic process. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro; UBE2D4 able to promote polyubiquitination using all 7 ubiquitin Lys residues; but may prefer 'Lys-11' and 'Lys-48'-linked poly-ubiquitination.