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Recombinant Human UBE2G2 Protein (GST Tag)
SKU: PKSH033184-50
Recombinant Human UBE2G2 Protein (GST Tag)
| SKU # | PKSH033184 |
| Expression Host | E.coli |
Description
| Synonyms | UBE2G2, Ubiquitin Carrier Protein G2, Ubiquitin-Conjugating Enzyme E2 G2, Ubiquitin-Protein Ligase G2 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Leu165 |
| Accession | P60604 |
| Calculated Molecular Weight | 45.0 kDa |
| Observed Molecular Weight | 43 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
| Reconstitution | Not Applicable |
Background
Ubiquitin-Conjugating Enzyme E2 G2 (UBE2G2) is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation, which belong to the ubiquitin-conjugating enzyme family. It shares 60% and 100% sequence identity with S.cerevisiae Ubc7 and mouse respectively. The UBE2G2 enzyme and the GP78 E3 ligase are active components of endoplasmic reticulum-associated degradation pathway which is essential for the degradation of misfolded ER proteins. The mechanism of K48-linked poly-ubiquitination by UBE2G2/GP78 appears to involve the transfer of preassembled Ub chains from UBE2G2 to lysine residues in a substrate. The E2 and E3 enzymes form a large hetero-oligomer which brings multiple UBE2G2 molecules into close proximity which allows for Ub transfer between neighboring E2s.