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Recombinant Human UBE2I/UBC9 Protein (GST Tag)
SKU: PKSH033308-50
Recombinant Human UBE2I/UBC9 Protein (GST Tag)
| SKU # | PKSH033308 |
| Expression Host | E.coli |
Description
| Synonyms | SUMO-Conjugating Enzyme UBC9, SUMO-Protein Ligase, UBC9, UBCE9, UBE2I, Ubiquitin Carrier Protein 9, Ubiquitin Carrier Protein I, Ubiquitin-Conjugating Enzyme E2 I, Ubiquitin-Protein Ligase I, p18 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Ser158 |
| Accession | P63279 |
| Calculated Molecular Weight | 44.4 kDa |
| Observed Molecular Weight | 40 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, pH 7.5. |
| Reconstitution | Not Applicable |
Background
SUMO-Conjugating Enzyme UBC9 (UBC9) belongs to the ubiquitin-conjugating enzyme family. UBC9 is homologous to ubiquitin-conjugating enzymes (E2s). However; instead of conjugating ubiquitin; UBC9 conjugates a ubiquitin homologue; Small Ubiquitin-Like Modifier 1 (SUMO-1). The conjugation of ubiquitin requires the activities of ubiquitin-activating (E1) and conjugating (E2) enzymes. It is suggested that UBC9 might play a role in DNA repair and perhaps even in aging.