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Recombinant Human USP14 Protein (His Tag)
SKU: PKSH033174-50
Recombinant Human USP14 Protein (His Tag)
| SKU # | PKSH033174 |
| Expression Host | E.coli |
Description
| Synonyms | Deubiquitinating Enzyme 14, TGT, USP14, Ubiquitin Carboxyl-Terminal Hydrolase 14, Ubiquitin Thioesterase 14, Ubiquitin-Specific-Processing Protease 14 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Asp91-Gln494 |
| Accession | P54578 |
| Calculated Molecular Weight | 48.5 kDa |
| Observed Molecular Weight | 52 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 20% Glycerol, pH 8.0. |
| Reconstitution | Not Applicable |
Background
Ubiquitin Carboxyl-Terminal Hydrolase 14 (USP14) belongs to the ubiquitin-specific processing (USP) family which is a deubiquitinating enzyme (DUB) with His and Cys domains. USP14 located in the cytoplasm is a proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. USP14 acts also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. In addition, USP14 is indispensable for synaptic development and function at neuromuscular junctions, required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis.