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Recombinant Human Zic3 protein (His tag)
SKU: PDEH100321-100
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Recombinant Human Zic3 protein (His tag)
| SKU # | PDEH100321 |
| Expression Host | E. coli |
Description
| Synonyms | Zinc finger protein ZIC 3;ZIC3;Zinc finger protein 203;Zinc finger protein of the cerebellum 3 |
| Species | Human |
| Expression_host | E.coli |
| Sequence | Met 1-Val467 |
| Accession | O60481 |
| Mol_Mass | 51.3 kDa |
| AP_Mol_Mass | 52 kDa |
| Tag | N-His & C-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
ZIC3 (zinc-finger protein of the cerebellum 3) is a 50 kDa (predicted) member of the GLI C2H2-type Zn-finger protein family. It is expressed in cerebellum, and apparently plays a key role in left-right body axis formation. Human ZIC3 is 467 amino acids (aa) in length. It contains N?terminal poly-Ala (aa 46?55) and poly-His (aa 87?97) segments, followed by five C2H2-type Zn-fingers (aa 253?410). Within the zinc finger region, there is an overlapping NES (aa 290?349) and two NLS's (aa 367?382 and 403?410). ZIC3 reportedly binds to GLI-3.