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Recombinant Mouse AFP Protein (His Tag)
SKU: PDEM100030-100
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Recombinant Mouse AFP Protein (His Tag)
| SKU # | PDEM100030 |
| Expression Host | E. coli |
Description
| Synonyms | Afp;AFPD;Alpha fetoglobulin;Alpha fetoprotein;Alpha fetoprotein precursor;Alpha-1-fetoprotein;Alpha-fetoglobulin;Alpha-fetoprotein;alpha-fetoprotein;Hereditary persistence of;included;FETA;FETA;Hereditary persistence of alpha fetoprotein;HPAFP |
| Species | Mouse |
| Expression_host | E.coli |
| Sequence | Tyr273-Val605 |
| Accession | P02772-1 |
| Mol_Mass | 25.9 kDa |
| AP_Mol_Mass | 28.2 kDa |
| Tag | N-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.