Recombinant Mouse MME/CD10/Neprilysin protein (His tag)
SKU: PDEM100075-100
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Recombinant Mouse MME/CD10/Neprilysin protein (His tag)
SKU # | PDEM100075 |
Expression Host | E. coli |
Description
Synonyms | Atriopeptidase;CALLA;Enkephalinase;EPN;NEP;Neprilysin;neprilysin-390;neprilysin-411;SFE;Skin fibroblast elastase |
Species | Mouse |
Expression_host | E.coli |
Sequence | Met 461-Trp 750 |
Accession | Q61391 |
Mol_Mass | 31.8 kDa |
AP_Mol_Mass | 32 kDa |
Tag | N-His |
Bio_Activity | Not validated for activity |
Properties
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin level | Please contact us for more information. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
CD10, also known as neprilysin, common acute lymphoblastic leukemia antigen (CALLA), or neutral endopeptidase (NEP), is a zinc-dependent transmembrane metallo-endopeptidase. CD10 cleaves peptides at the N-terminal side of hydrophobic amino acid residues and deactivates a variety of signaling peptides. CD10 was shown to be one of the markers for a natural killer cell-restricted progenitor in fetal and adult tissues. Studies also suggest CD10 as a phenotypic marker to distinguish mature neutrophils from immature neutrophils in patients with inflammation. In addition, CD10+GPR77+ carcinoma-associated fibroblasts (CAFs) promote tumor formation and chemoresistance.