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Recombinant Rat Prostatic Acid Phosphatase/ACPP Protein (His tag)
SKU: PDER100086-100
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Recombinant Rat Prostatic Acid Phosphatase/ACPP Protein (His tag)
| SKU # | PDER100086 |
| Expression Host | E. coli |
Description
| Synonyms | Acid phosphatase 3, Acp3, Fluoride-resistant acid phosphatase, Prostatic acid phosphatase, Protein tyrosine phosphatase ACP3, Thiamine monophosphatase (TMPase) |
| Species | rat |
| Expression Host | E.coli |
| Sequence | Lys 32-Leu 381 |
| Accession | P20646 |
| Calculated Molecular Weight | 38.4 kDa |
| Observed Molecular Weight | 43 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
ACPP (Acid phosphatase, prostate; also PAP and ACP3) is a 48-52 kDa glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95-100 kDa nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP.