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Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD (C-mFc-6His tag) (Active)
SKU: PKSV030276-100
Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD (C-mFc-6His tag) (Active)
| SKU # | PKSV030276 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | S protein RBD;Spike glycoprotein Receptor-binding domain;S glycoprotein RBD;Spike protein RBD;COVID-19 |
| Species | SARS-CoV-2 |
| Expression_host | HEK293 Cells |
| Sequence | Arg319-Phe541 |
| Accession | QHD43416.1 |
| Mol_Mass | 52.2 kDa |
| Tag | C-mFc-His |
| Bio_Activity | 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human S-RBD, mFc-His tagged protein (PKSV030276) can bind Anti-SARS-CoV-2 Neutralizing antibody(E-AB-V1028) in a linear range of 0.24-15.62 ng/ml. 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, mFc-His tagged protein (PKSV030276) can bind Human ACE2, hFc Tagged protein(PKSR030492) in a linear range of 0.488-49.83 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.