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Sodium (Na) Colorimetric Assay Kit
SKU: E-BC-K207-M-96
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Sodium (Na) Colorimetric Assay Kit
| SKU # | E-BC-K207-M |
| Detection Instrument | Microplate reader (405 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Sample Type | Serum, plasma, tissue |
| Sensitivity | 0.02 mmol/L |
| Detection Range | 0.02 - 20 mmol/L |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 15 min |
| Precision | Average inter-assay CV: 8.400% | Average intra-assay CV: 2.200% |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of Sample
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| Human serum | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| Rat plasma | 1 |
Note: The diluent is double distilled water. For the dilution of other sample types, please do pretest to confirm the dilution factor
Detection Principle
β-galactosidase activated by sodium ions can catalyze o-nitrophenol to produce glycoside and o-nitrophenol. The increase of absorbance is determined at 405 nm, and the content of sodium ion is calculated indirectly.
Kit Components & Storage
| Item | Component | Size 1(48 T) | Size 2(96 T) | Storage |
| Reagent 1 | Chromogenic Agent | 5 mL × 1 vial | 10 mL × 1 vial | 2-8℃, 12 months shading light |
| Reagent 2 | Enzyme Stock Solution | 10 mL × 1 vial | 20 mL × 1 vial | 2-8℃, 12 months |
| Reagent 3 | Enzyme Reagent | Power × 1 vial | Power × 2 vials | 2-8℃, 12 months |
| Reagent 4 | 10 mmol/L Standard | 1.6 mL ×1 vial | 1.6 mL ×1 vial | 2-8℃, 12 months |
| Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 2.50 | 9.70 | 15.60 |
| %CV | 2.5 | 2.1 | 2.0 |
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 2.50 | 9.70 | 15.60 |
| %CV | 8.2 | 8.5 | 8.5 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 98%.
| Sample 1 | Sample 2 | Sample 3 | |
| Expected Conc. (mmol/L) | 4.6 | 12.5 | 17.3 |
| Observed Conc. (mmol/L) | 4.6 | 11.8 | 17.5 |
| Recovery rate (%) | 99 | 94 | 101 |
Sensitivity
The analytical sensitivity of the assay is0.02 mmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.