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Sodium (Na+) Colorimetric Assay Kit
SKU: E-BC-K207-S-200
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Sodium (Na+) Colorimetric Assay Kit
| SKU # | E-BC-K207-S |
| Detection Instrument | Spectrophotometer (405 nm), Microplate reader (405 nm), Biochemistry analyzer (405 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Synonyms | Na |
| Sample Type | Serum |
| Detection Range | 80-180 mmol/L |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Storage | 2-8℃ |
| Valid period | 12 months |
Images
M Podder et al investigate the physiological, blood-biochemical and behavioural changes of Ghoongroo pigs in seasional heat stress. Sodium (Na) concentration in pig serum was determined using sodium colorimetric assay kit (E-BC-K207-S).
Table. Blood variables of Ghoongroo pigs in different physiological groups in different seasons.
| Item | Group (G) | Season1 (S) | Group mean | SEM | P value | ||||
| Summer | Rainy | Winter | Group | Season | GxS | ||||
| Erythrocyte (x 109/mL) | Boar | 9.47a | 8.03b,x | 5.00c | 7.5 | 0.12 | <0.001 | <0.001 | <0.001 |
| Non-pregnant sow | 9.38a | 5.81b,y | 5.52b | 6.9 | |||||
| Pregnant sow | 9.38a | 6.30b,y | 5.48c | 7.05 | |||||
| Season mean | 9.41 | 6.71 | 5.33 | ||||||
| Leukocyte (x 106/mL) | Boar | 16.8a | 15.3a,y | 11.9b,z | 14.7 | 0.45 | <0.001 | <0.001 | 0.001 |
| Non-pregnant sow | 18.3 | 17.5x | 17.8x | 17.8 | |||||
| Pregnant sow | 18.3a | 18.4a,x | 15.1b.y | 17.3 | |||||
| Season mean | 17.8 | 17.1 | 14.9 | ||||||
| Haemoglobin (g/dL) | Boar | 16.0a,x | 13.1b | 11.0c | 13.4 | 0.44 | 0.019 | <0.001 | 0.001 |
| Non-pregnant sow | 12.0y | 12.6 | 12.5 | 12.4 | |||||
| Pregnant sow | 12.1b,y | 14.4a | 11.9b | 12.8 | |||||
| Season mean | 13.4 | 13.4 | 11.8 | ||||||
| Cortisol (µg/dL) | Boar | 9.00a | 8.44b,y | 8.40b,xy | 8.61 | 0.03 | <0.001 | <0.001 | 0.0201 |
| Non-pregnant sow | 8.90a | 8.40b,y | 8.30b,y | 8.53 | |||||
| Pregnant sow | 8.95a | 8.60b,x | 8.50b,x | 8.68 | |||||
| Season mean | 8.95 | 8.48 | 8.4 | ||||||
| Sodium (mEq/L) | Boar | 134 | 146 | 151 | 144 | 1.54 | 0.35 | <0.001 | 0.51 |
| Non-pregnant SOW | 135 | 145 | 147 | 142 | |||||
| Pregnant sow | 134 | 147 | 147 | 143 | |||||
| Season mean | 135b | 146a | 148a | ||||||
| Potassium (mEq/L) | Boar | 4.58 | 5.62 | 6.11 | 5.44 | 0.18 | 0.34 | <0.001 | 0.94 |
| Non-pregnant sow | 4.5 | 5.5 | 5.74 | 5.25 | |||||
| Pregnant sow | 4.43 | 5.52 | 5.79 | 5.25 | |||||
| Season mean | 4.50c | 5.55b | 5.88a | ||||||
Sodium concentration in pig serum during winter is higher than that in summer.
Detection Principle
Production of o-nitrophenol and galactose by o-nitrophenol-β-D-galactoside (ONPG) catalyzed by sodium dependent β-D-galactosidase. The amount of o-nitrophenol is directly proportional to the concentration of sodium ion in the sample. The o-nitrophenol is yellow in alkaline environment. The increase of absorbance is determined at 405 nm, and the content of sodium ion is calculated indirectly.
Kit Components & Storage
| Item | Component | Specification | Storage |
| Reagent 1 | Tris-HCl Buffer Solution | 20 mL × 2 vials | 2-8℃,12 months, shading light |
| β- Galactosidase | |||
| Cryptand | |||
| Reagent 2 | ONPG | 10 mL × 2 vials | |
| Tris-HCl Buffer Solution | |||
| Reagent 1 | 140 mmol/L Standard | 2 mL × 1 vial |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.