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Total Phenols Colorimetric Assay Kit (Plant Samples)
SKU: E-BC-K354-S-200
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Total Phenols Colorimetric Assay Kit (Plant Samples)
| SKU # | E-BC-K354-S |
| Detection Instrument | Spectrophotometer (760 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Sample type | Plant tissue |
| Sensitivity | 0.73 μg/mL |
| Detection range | 0.73-150 μg/mL |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 60 min |
| Precision | Average inter-assay CV: 2.500% | Average intra-assay CV: 1.900% |
| Other instruments required | Micropipettor, Vacuum dryer, Vortex mixer, Ultrasonic cell grinder, Crusher |
| Other reagents required | 60% Ethanol |
| Storage | 2-8℃ |
| Valid period | 12 months |
Detection Principle
Under alkaline conditions, tungsten-molybdenum acid can be reduced by phenols and produce blue compounds, which has a characteristic absorption peak at 760 nm. The content of total phenols in sample can be calculated indirectly by measuring the absorbance at 760 nm.
Kit Components & Storage
| Item | Component |
Size 1 (100 assays) |
Size 2 (200 assays) |
Storage |
| Reagent 1 | Folin Phenol Reagent | 60 mL × 1 vial | 60 mL × 2 vial | 2-8°C, 12 months shading light |
| Reagent 2 | Alkali | Powder × 2 vials | Powder × 4 vials | 2-8°C, 12 months |
| Reagent 3 | O-dihydroxybenzene | 10 mg ×4 vials | 10 mg × 8 vials | 2-8°C, 12 months shading light |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (μg/mL) | 9.40 | 48.50 | 96.20 |
| %CV | 2.3 | 1.8 | 1.6 |
Inter-assay Precision
Three human serum samples were assayed 17 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (μg/mL) | 9.40 | 48.50 | 96.20 |
| %CV | 2.1 | 2.6 | 2.8 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 101%.
| Standard 1 | Standard 2 | Standard 3 | |
| Expected Conc. (μg/mL) | 32.5 | 84.5 | 126 |
| Observed Conc. (μg/mL) | 32.2 | 87.0 | 127.3 |
| Recovery rate (%) | 99 | 103 | 101 |
Sensitivity
The analytical sensitivity of the assay is 0.73 μg/mL. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.
Standard Curve
As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:
| Concentration (μg/mL) | 0 | 40 | 60 | 80 | 100 | 120 | 150 |
| Average OD | 0.001 | 0.209 | 0.309 | 0.412 | 0.506 | 0.599 | 0.737 |
| Absoluted OD | 0 | 0.208 | 0.308 | 0.411 | 0.505 | 0.598 | 0.736 |