Accuris QuickSilver™ Powdered Buffer Packs (TAE, TBS, PBS, etc.)

Premeasured pouches make 1 liter of 1X buffer solution
Just empty pouch into water and stir – no diluting of stock solutions
No pH adjustment necessary
Quality and consistency guaranteed
Nuclease and protease free
Long shelf life

Electrophoresis Buffers:

TAE
Tris-Glycine-SDS
Tris-MES-SDS

Western Blotting, ELISA and Cell Culture:

TBS
TBS-T
PBS
PBS-T

Applications

Nucleic Acid Electrophoresis Buffer

QuickSilver™ TAE (Tris-acetate-EDTA)

TAE is ideal for separation of large DNA fragments in agarose gels and is used as running buffer and in gel preparation.

TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions. Final concentration of a 1X working solution is 40mM Tris, 20mM acetic acid, 1mM EDTA.

EB1000 QuickSilver TAE Buffer, 1X, 100 packs

Protein Electrophoresis Buffers

QuickSilver™ TGS (Tris-Glycine-SDS)

Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.

EB1100 QuickSilver Tris Glycine-SDS Buffer, 1X, 50 packs

QuickSilver™ Tris-MOPS-SDS

Tris-MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with Tris-MOPS-SDS buffer run slower than those using Tris-MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.

EB1102 QuickSilver Tris-MOPS-SDS, 1X, 50 packs

Western Blotting, ELISA, Cell Culture, Chromatography

QuickSilver™ PBS and PBST

Because its osmolality and ion concentration closely mimic that of the human body, PBS is commonly used for cell culture applications, as well as HPLC and MS. PBST is a wash buffer and diluent for ELISA. The 1X PBS solution contains 137mN NaCl, 2.7mM KCl and 10mM phosphate. PBST is supplemented with 0.05% Tween-20.

EB1200 QuickSilver PBS Buffer, 1X, 100 packs

EB1201 QuickSilver PBST Buffer, 1X, 100 packs

QuickSilver™ TBS and TBST

TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.

EB1202 QuickSilver TBS buffer, 1X, 100 packs

EB1203 QuickSilver TBST buffer, 1X, 100 packs

Ordering Information

SKU #	Description
EB1000	QuickSilver TAE Buffer Powder, 100 pouches
EB1100	QuickSilver Tris-Glycine-SDS Buffer Powder, 50 pouches
EB1102	QuickSilver Tris-MOPS-SDS Buffer Powder, 50 pouches
EB1200	QuickSilver PBS Buffer Powder, 100 pouches
EB1201	QuickSilver PBS-T Buffer Powder, 100 pouches
EB1202	QuickSilver TBS Buffer Powder, 100 pouches
EB1203	QuickSilver TBS-T Buffer Powder, 100 pouches

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