Accuris SmartCheck DNA Ladder

Accuris SmartCheck™ DNA Ladder

A DNA ladder is a solution of DNA molecules of different lengths used in agarose or acrylamide gel electrophoresis. It is applied as a reference to estimate the size of unknown DNA molecules that were separated based on their mobility in an electrical field through the gel.

• Ready-to-use formulation includes loading buffer and tracking dye
• 500µL suitable for 100 lanes (5µL per lane)
• Higher intensity reference bands
• Ultra pure production allows economical ambient shipping

Technical Details

• Protocol: Briefly vortex the tube and use 5µL per lane. Additional loading buffer is not required.
• Concentration: 0.1 mg/mL
• Tracking dye: bromophenol blue and xylene cyanol
• Buffer formulation: 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol
• Shipping and Storage: SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum stability and long-term storage up to 15 months.
• Production: SmartCheck™ ladders are produced from proprietary plasmids, digested to completion. A multistep chromatography method is used to ensure purity and DNA quality.
• Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.

Specifications

MSE supplies is an authorized distributor of Accuris products and accessories.