Anti-DYKDDDDK-tag FAST IP kit

Anti-DYKDDDDK-tag FAST IP kit

Background

Anti-DYKDDDDK-tag fast IP kit is composed of Anti-DYKDDDDK affinity gel, Mouse IgG affinity gel, DYKDDDDK-Tag Rabbit pAb and Goat Anti-Rabbit IgG(peroxidase/HRP conjugated), which is used for fast, efficient and specific (Co)IP of DYKDDDDK tag fusion protein.

Anti-DYKDDDDK affinity gel is made by covalent coupling of high-quality DYKDDDDK-Tag Mouse mAb and Sepharose 4B agarose granules. It has the characteristics of high binding capacity of protein, high specificity and stability. Mouse IgG affinity gel is used as the control for CoIP experiment, and its properties are stable. The DYKDDDDK-Tag Rabbit pAb antibody has the advantages of high specificity, high affinity and high valence; Goat Anti-Rabbit IgG (peroxidase/HRP conjugated) is purified by cross-adsorption and only DYKDDDDK-Tag Rabbit pAb is identified, and there is no cross-reaction with the heavy-chain and light-chain of the mouse monoclonal antibody. The four components are strictly inspected and can be used independently; the combination of four sets has the advantages of fast, convenience and no interference band.

Performance

1.       Scope of application

(Co)IP of the DYKDDDDK tag fusion protein.

DYKDDDDK tag can be located at the N-terminal, C-terminal or middle of the protein, such as N-terminal DYKDDDDK fusion protein (DYKDDDDK-Protein), C-terminal DYKDDDDK fusion protein (Protein-DYKDDDDK) and the Met-modified N-terminal DYKDDDDK fusion protein (Met-DYKDDDDK-Protein).

2.       Antibody properties

DYKDDDDK-Tag Mouse mAb: Mouse IgG2a subtype;

DYKDDDDK-Tag Rabbit pAb: Rabbit IgG.

3.       Gel properties

Agarose gel granules, average size 50μm.

4.         Binding capacity

0.5mL Sepharose 4B agarose granules, covalently coupled with 4mg Anti-DYKDDDDK mouse monoclonal antibody.

Product Components

* Note: Buffer is PBS containing 50% glycerol.

Storage

-20°C, 12 months.