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Anti-GFP Nanobody Affinity Agarose, 2mL
SKU: EA-IP-004N
Anti-GFP Nanobody Affinity Agarose, 2mL
Background
GFP, a green fluorescent protein, is derived from Aequorea victoria multi tube luminous jellyfish and is composed of about 238 amino acids. It can stimulate green fluorescence from blue light to ultraviolet light. GFP is often used as a marker for recombinant expression of eukaryotic proteins. Anti-GFP Nanobody affinity gel is made by covalently coupling high-quality GFP nanobody with agarose gel. As the nanobody only contains the variable region of antibody molecules, there will be no signal interference from the heavy and light chains of the antibody during immuno (co) precipitation. It has the characteristics of high binding capacity of protein, high specificity and stability, and can be used for immuno (co) precipitation of GFP tagged fusion proteins.
Performance Index
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Scope of application |
Immuno (co) precipitation of GFP tag fusion protein. The GFP tag can be located at the N-terminal or C-terminal of the protein, such as the N-terminal GFP fusion protein (GFP–Protein) and the C-terminal GFP fusion protein (Protein-GFP). |
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Antibody properties |
Anti-GFP Nanobody. |
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Gel Properties |
Agarose gel granules, average size 50 μm. |
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Binding capacity |
1mL Sepharose 4B agarose granules are covalently coupled with 6mg Anti-GFP Nanobody. 1mL affinity gel can precipitate at least 1.2mg GFP fusion protein. |
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Components |
1mL Anti-GFP affinity gel, stored in 1mL PBS containing preservative and 50% glycerol. |
Storage
-20°C, 12 months.