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Anti-mCherry Nanobody Affinity Agarose, 2mL
SKU: EA-IP-005N
Anti-mCherry Nanobody Affinity Agarose, 2mL
Background
mCherry, a red fluorescent protein derived from sea anemone (Discosoma sp.), is composed of 266 amino acids. Because of its color and light stability of monomer molecules, it performs better than other fluorescent protein tags. mCherry is often used as eukaryotic protein recombinant expression marker. Anti-mCherry nanobody affinity gel is made by covalently coupling high-quality mCherry nanobody with agarose gel. As the nanobody only contains the variable region of antibody molecules, there will be no signal interference from the heavy and light chains of the antibody during immuno (co) precipitation. It has the characteristics of high binding capacity of protein, high specificity and stability, and can be used for immuno (co) precipitation of mCherry tagged fusion protein.
Performance Index
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Scope of application |
Immuno (co) precipitation of mCherry tag fusion protein. The mCherry tag can be located at the N-terminal and C-terminal of the protein, such as the N-terminal mCherry fusion protein (mCherry–Protein) and the C-terminal mCherry fusion protein (Protein-mCherry). |
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Antibody properties |
Anti-mCherry Nanobody. |
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Gel properties |
Agarose gel granules, average size 50 μm. |
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Binding capacity |
1mL Sepharose 4B agarose granules are covalently coupled with 6mg Anti-mCherry Nanobody. 1mL affinity gel can precipitate at least 1.2mg mCherry fusion protein |
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Components |
1mL Anti-mCherry affinity gel, stored in 1mL PBS containing preservative and 50% glycerol. |
Storage
-20°C, 12 months.