Bioneer AccuPower Pfu PCR PreMix (96 T, 20 μl)
SKU: K-2022
Bioneer AccuPower® Pfu PCR PreMix (96 T, 20 μl)
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AccuPower® Pfu PCR PreMix uses Pfu DNA polymerase to attain highly accurate PCR products through its proof-reading function. As all components necessary for PCR such as Pfu DNA polymerases, dNTPs, reaction buffers, etc., are thoroughly mixed and vacuum-dried, each packaged with amounts sufficient for a single PCR run, by simply adding the template DNA and primers, a high-performance PCR product can be obtained.
※ PreMix product is shipped at room temperature.
※ The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
※ Master Mix product is shipped in dry ice.
Features and Benefits
High Fidelity
Low mutation rate during DNA amplification through its a high fidelity (error rate= 1.9X10-6).
Sensitivity
Excellent sensitivity and amplification efficiency by detecting even a trace amount of samples.
Long Range PCR
Effective amplification of large-size targets allowing various applications such as promoter assay and cloning experiments.
Stability
Enhanced stability allowing long-term storage with a stabilizer included and dried in the PCR reaction mixture.
User-friendly
Convenance with easy start just by adding template DNA and primers.
Reproducibility
Minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variable by mass production under ISO 9001 quality system.
Applications
- Gene synthesis
- Gene cloning
- Conventional PCR
- Primer extension
- Site-directed mutagenesis
- High fidelity PCR
Specifications
Enzyme | Pfu DNA polymerase |
5' to 3' exonuclease | No |
3' to 5' exonuclease | Yes |
3' – A overhang | No |
Fragment Size | Up to 15 kb |