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Bioneer MagListo 5M Plant Genomic DNA Extraction Kit (100 reactions)
SKU: K-3605
Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit (100 reactions)
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MagListo™ 5M Plant Genomic DNA Extraction Kit allows extraction of total DNA using Magnetic Nanobeads and MagListo™ Magnetic Separation Rack from various plant samples such as leaves, roots, seeds. The isolated genomic DNA can be used for a wide range of experiments such as gene cloning, PCR, Quantitative real time PCR, southern blotting, etc. Magnetic bead type.
Features and Benefits
Flexible Sample Choice
Isolation of highly-pure genomic DNA from plant samples such as leaves, stem, roots, seeds with high-yield
Great Lysis Optimization
Lysis Buffer included in the kit for various plant seeds and tissue cells
Convenience
Broad coverage of scales for Mini, Midi, and Maxi isolation protocols with just a single kit (Mini - within 5min, Midi - within 10min, Maxi - within 15min)
Cost-effective Kit
No need of additional expensive instruments other than the kit and MagListo™ Magnetic Separation Rack
Applications
- Gene cloning
- PCR
- Real-time PCR
- Southern blotting
- SNP genotyping
Specifications
| Scale | Mini (typical yield (μg)) |
Midi (typical yield (μg)) |
Maxi (typical yield (μg)) |
| Plant tissue | Up to 100 mg | Up to 500 mg | Up to 1 g |
| Seed | Up to 50 mg | Up to 250 mg | Up to 500 mg |
| Elution volume | 100 ul | 500 ul | 1 ml |
| Typical DNA yield* | 5 - 15 ug | 15 - 80 ug | 30 - 150 ug |
| Expected purity | A260/280 > 1.7 | ||
* Differences in the quantified value may occur depending on the types of samples.
Components
| Components | K-3605* |
| PL Buffer | 35 ml x 1 ea |
| PC Buffer | 30 ml x 1 ea |
| PWM1 Buffer | 60 ml x 1 ea |
| W2 Buffer | 80 ml x 1 ea |
| WE Buffer | 120 ml x 1ea |
| EA Buffer | 25 ml x 1 ea |
| RNase A powder, lyophilized | 24 mg x 2 ea |
| Proteinase K, lyophilized | 25 mg x 2 ea |
| Magnetic Nanobead | 11 ml x 1 ea |
| User's Guide | 1 ea |
*mini-100 rxn, midi-15 rxn, maxi-8 rxn